abstract：:Transcription of the human interphotoreceptor retinoid binding protein (IRBP) gene is strictly tissue specific, being restricted to retinal photoreceptors and pinealocytes. We have previously demonstrated that a sequence named A element, in the IRBP promoter is essential for IRBP gene transcription in vivo. Here we de...

journal_title：Mechanisms of development

authors： Bobola N,Briata P,Ilengo C,Rosatto N,Craft C,Corte G,Ravazzolo R

更新日期：1999-04-01 00:00:00

abstract：:In the Drosophila embryo the nuclear localisation of Dorsal, a member of the Rel family, is regulated by an extracellular signal, which is transmitted to the interior of the egg cell by a cascade of proteins involving the novel protein Tube and the protein kinase Pelle. Here we analyse the activation mechanism of Tube...

journal_title：Mechanisms of development

authors： Grosshans J,Schnorrer F,Nüsslein-Volhard C

更新日期：1999-03-01 00:00:00

abstract：:We report the expression pattern of a murine homolog of the Xenopus laevis T-box gene Eomesodermin. mEomes expression is first detected in the extra-embryonic ectoderm prior to gastrulation, and persists there until head-fold stages. In the embryo proper, mEomes is expressed throughout the early primitive streak, nasc...

journal_title：Mechanisms of development

authors： Ciruna BG,Rossant J

更新日期：1999-03-01 00:00:00

abstract：:The Extramacrochaetae (emc) gene encodes a transcription factor with an HLH domain without the basic region involved in interaction with DNA present in other proteins that have this domain. EMC forms heterodimers with bHLH proteins preventing their binding to DNA, acting as a negative regulator. The function of emc is...

journal_title：Mechanisms of development

authors： Baonza A,García-Bellido A

更新日期：1999-02-01 00:00:00

abstract：:We have sequenced and defined the expression during rat embryogenesis of the protocadherin fat, the murine homologue of a Drosophila tumour suppressor gene. As previously described for human fat, the sequence encodes a large protocadherin with 34 cadherin repeats, five epidermal growth factor (EGF)-like repeats contai...

journal_title：Mechanisms of development

authors： Ponassi M,Jacques TS,Ciani L,ffrench Constant C

更新日期：1999-02-01 00:00:00

abstract：:The forkhead type Brain Factor 2 from mouse and chicken help pattern the forebrain, optic vesicle and kidney. We have isolated a Xenopus homolog (Xbf2) and found that during gastrulation it is expressed in the dorsolateral mesoderm, where it helps specify this territory by downregulating BMP-4 and its downstream genes...

journal_title：Mechanisms of development

authors： Gómez-Skarmeta JL,de la Calle-Mustienes E,Modolell J,Mayor R

更新日期：1999-01-01 00:00:00

abstract：:Mll, Brg1 and Brm are vertebrate homologues of Drosophila trithorax group (trxG) genes. We isolated chicken Mll cDNA clones, and examined patterns of Mll, Brg1 and Brm expression in chick embryos. All three genes were expressed from embryonic stage 2 onwards. Mll transcripts were just detectable in all tissues by in s...

journal_title：Mechanisms of development

authors： Schofield J,Isaac A,Golovleva I,Crawley A,Goodwin G,Tickle C,Brickell P

更新日期：1999-01-01 00:00:00

abstract：:In multicellular organisms, very little is known about the role of mRNA stability in development, and few proteins involved in degradation pathways have been characterized. We have identified the Drosophila homologue of XRN1, which is the major cytoplasmic 5'-3' exoribonuclease in Saccharomyces cerevisiae. The protein...

journal_title：Mechanisms of development

authors： Till DD,Linz B,Seago JE,Elgar SJ,Marujo PE,Elias ML,Arraiano CM,McClellan JA,McCarthy JE,Newbury SF

更新日期：1998-12-01 00:00:00

abstract：:Mice with targeted disruption of the TGF beta 2 gene display defects in epithelial-mesenchymal tissue interactions in several tissues including the developing cochlea. Specifically, the region of the spiral limbus and the overlying interdental cells, structures putatively involved in endolymphatic fluid homeostasis, d...

journal_title：Mechanisms of development

authors： Paradies NE,Sanford LP,Doetschman T,Friedman RA

更新日期：1998-12-01 00:00:00

abstract：:We have isolated and characterized two complete cDNA clones, Zfz8a and Zfz8b, which encode zebrafish Frizzled (Fz) homologues. The predicted protein sequences, spanning 579 and 576 amino acid residues for ZFz8a and ZFz8b, respectively, were highly homologous (78%) to each other and contained an extracellular cysteine-...

journal_title：Mechanisms of development

authors： Kim SH,Park HC,Yeo SY,Hong SK,Choi JW,Kim CH,Weinstein BM,Huh TL

更新日期：1998-11-01 00:00:00

abstract：:In the Drosophila embryo, a subset of muscles require expression and function of the RYK subfamily RTK gene derailed (drl) for correct attachment. We have isolated a second RYK homolog, doughnut (dnt), from Drosophila. The DNT protein exhibits 60% amino acid identity to DRL, and is structurally as similar to the mamma...

journal_title：Mechanisms of development

authors： Oates AC,Bonkovsky JL,Irvine DV,Kelly LE,Thomas JB,Wilks AF

更新日期：1998-11-01 00:00:00

abstract：:The Fused (Fu) serine-threonine kinase and the Suppressor of fused (Su(fu)) product are part of the Hedgehog (Hh) signaling pathway both in embryos and in imaginal discs. In wing imaginal discs, the Hh signal induces Cubitus interruptus (Ci) accumulation and activates patched (ptc) and decapentaplegic (dpp) expression...

journal_title：Mechanisms of development

authors： Alves G,Limbourg-Bouchon B,Tricoire H,Brissard-Zahraoui J,Lamour-Isnard C,Busson D

更新日期：1998-11-01 00:00:00

abstract：:In a large-scale gene expression screen 1765 randomly picked cDNAs were analyzed by whole-mount in situ hybridization in Xenopus embryos. Two hundred and seventy three unique, differentially expressed genes were identified, 204 of which are novel in Xenopus. Partial DNA sequences and expression patterns were documente...

journal_title：Mechanisms of development

authors： Gawantka V,Pollet N,Delius H,Vingron M,Pfister R,Nitsch R,Blumenstock C,Niehrs C

更新日期：1998-10-01 00:00:00

abstract：:Members of the cysteine-rich protein (CRP) define a subclass of LIM-only proteins implicated mainly in muscle differentiation. Until now, very little is known concerning the expression of CRP encoding genes during vertebrate development. We describe here the isolation of a trout (Oncorhynchus mykiss) gene encoding a c...

journal_title：Mechanisms of development

authors： Delalande JM,Rescan PY

更新日期：1998-08-01 00:00:00

abstract：:We investigated the expression pattern of the endothelin-A receptor and endothelin 1 genes, the mutations of which affect the development of the mesectodermal derivatives of the neural crest. We show here that endothelin 1 is expressed by the environment of the cephalic neural crest cells invading branchial arches. La...

journal_title：Mechanisms of development

authors： Nataf V,Grapin-Botton A,Champeval D,Amemiya A,Yanagisawa M,Le Douarin NM

更新日期：1998-07-01 00:00:00

abstract：:In chick embryos, naso-temporal polarity of the retina becomes established before Hamburger-Hamilton stage 10. To examine the plasticity of the early eye anlage, double-temporal eyes were made using stage 10-11 (E1.5) chick embryos and stage 8-9 quail embryos. In vivo and in vitro experiments revealed that these doubl...

journal_title：Mechanisms of development

authors： Mueller BK,Dütting D,Haase A,Feucht A,Macchi P

更新日期：1998-06-01 00:00:00

abstract：:To examine the role of the Oct-6 gene in Schwann cell differentiation we have cloned and characterized the chicken and zebrafish homologues of the mouse Oct-6 gene. While highly homologous in the Pit1-Oct1/2-Unc86 (POU) domain, sequence similarities are limited outside this domain. Both genes are intronless and both p...

journal_title：Mechanisms of development

authors： Levavasseur F,Mandemakers W,Visser P,Broos L,Grosveld F,Zivkovic D,Meijer D

更新日期：1998-06-01 00:00:00

abstract：:In mammals, 16 members of the Fgf family have so far been described with diverse roles in embryonic cell growth and differentiation. Here, we report the expression from early streak stage to midgestation of two newly-identified murine genes, Fgf17 and Fgf18, that are most closely related to Fgf8 (63.7% and 56.8% ident...

journal_title：Mechanisms of development

authors： Maruoka Y,Ohbayashi N,Hoshikawa M,Itoh N,Hogan BL,Furuta Y

更新日期：1998-06-01 00:00:00

abstract：:We have identified a short segment of the mouse Pax-6 gene 5' flanking region that is necessary and sufficient for reporter construct expression in components of the eye derived from non-neural ectoderm. This transcriptional control element has a highly conserved nucleotide sequence over 341 bp and is located approxim...

journal_title：Mechanisms of development

authors： Williams SC,Altmann CR,Chow RL,Hemmati-Brivanlou A,Lang RA

更新日期：1998-05-01 00:00:00

abstract：:The localization of RNAs at the vegetal cortex in Xenopus oocytes is a complex process, involving at least two different pathways. The early, or messenger transport organizer (METRO), pathway, localizes RNAs such as Xlsirts, Xcat2 and Xwnt11 during stages 1 and 2 of oogenesis, while the late pathway localizes RNAs suc...

journal_title：Mechanisms of development

authors： Kloc M,Etkin LD

更新日期：1998-04-01 00:00:00

abstract：:NK-2 class homeobox genes are candidate patterning and lineage regulators in diverse organisms. We report here the embryonic expression pattern of murine member, Nkx2-6. In keeping with its vertebrate relatives, Nkx2-6 was transcribed in ventrolateral embryonic structures. Expression was first detected at E8.0 in endo...

journal_title：Mechanisms of development

authors： Biben C,Hatzistavrou T,Harvey RP

更新日期：1998-04-01 00:00:00

abstract：:Msg1 and Mrg1 are founding members of a gene family which exhibit distinct patterns of gene expression during mouse embryogenesis. Sequence analysis reveals that these genes are unlike any other gene identified to date, but they share two near-identical sequence domains. The Msg1 and Mrg1 expression profiles during ea...

journal_title：Mechanisms of development

authors： Dunwoodie SL,Rodriguez TA,Beddington RS

更新日期：1998-03-01 00:00:00

abstract：:The chick dermis is known to control the formation of feathers and interfeathery skin in a hexagonal pattern. The evidence that the segregation of two types of fibroblasts involves Delta/Notch signalling is based on three facts. Rings of C-Delta-1-expressing fibroblasts precede and delimit the forming feather primordi...

journal_title：Mechanisms of development

authors： Viallet JP,Prin F,Olivera-Martinez I,Hirsinger E,Pourquié O,Dhouailly D

更新日期：1998-03-01 00:00:00

abstract：:Maleless (mle) is essential in Drosophila melanogaster males both in somatic cells and in germ cells. In somatic cells mle is necessary for X-chromosome dosage compensation. The role of mle in the germline is unknown. We have analyzed the expression pattern and localization of MLE, the other MSLs and acetylated isofor...

journal_title：Mechanisms of development

authors： Rastelli L,Kuroda MI

更新日期：1998-02-01 00:00:00

abstract：:XLPOU91, a POU-homeobox gene is expressed in a narrow window during early Xenopus development. We show that ectopic expression of XLPOU91 RNA causes severe posterior truncations in embryos without inhibiting the formation of Spemann's organizer. Ectopic XLPOU91 expression also inhibits mesoderm induction by fibroblast...

journal_title：Mechanisms of development

authors： Henig C,Elias S,Frank D

更新日期：1998-02-01 00:00:00

abstract：:The bHLH protein eHAND is a sensitive marker for cardiovascular precursors in the Xenopus embryo. The earliest site of expression is a broad domain within the lateral plate mesoderm of the tailbud embryo. This domain comprises precursors that contribute to the posterior cardinal veins in later stages. Surprisingly, ex...

journal_title：Mechanisms of development

authors： Sparrow DB,Kotecha S,Towers N,Mohun TJ

更新日期：1998-02-01 00:00:00

abstract：:This is the first in situ hybridization analysis of expression of a tumor necrosis factor (TNF) receptor associated factor (TRAF) during development. TRAF4 is observed throughout mouse embryogenesis, most notably during ontogenesis of the central (CNS) and peripheral (PNS) nervous system, and of nervous tissues of sen...

journal_title：Mechanisms of development

authors： Masson R,Régnier CH,Chenard MP,Wendling C,Mattei MG,Tomasetto C,Rio MC

更新日期：1998-02-01 00:00:00

abstract：:Cloning and sequencing of mouse Mf2 (mesoderm/mesenchyme forkhead 2) cDNAs revealed an open reading frame encoding a putative protein of 492 amino acids which, after in vitro translation, binds to a DNA consensus sequence. Mf2 is expressed at high levels in the ventral region of newly formed somites, in sclerotomal de...

journal_title：Mechanisms of development

authors： Wu SC,Grindley J,Winnier GE,Hargett L,Hogan BL

更新日期：1998-01-01 00:00:00

abstract：:The mouse and human Hox complex consists of 39 genes in four linkage groups (A-D). Although the structure and expression patterns of most of these genes have been reported, the 5' members of the Hox C linkage group have been only partially characterized. Herein we report the primary and genomic structure of the mouse ...

journal_title：Mechanisms of development

authors： Hostikka SL,Capecchi MR

更新日期：1998-01-01 00:00:00

abstract：:Splotch mice, which harbour mutations in the Pax3 gene, exhibit neural crest-related abnormalities including pigmentation defects, reduced or absent dorsal root ganglia and failure of cardiac outflow tract septation in homozygotes. Although splotch neural crest cells fail to colonise target tissues, they initiate migr...

journal_title：Mechanisms of development

authors： Henderson DJ,Ybot-Gonzalez P,Copp AJ

更新日期：1997-12-01 00:00:00

abstract：:Recent work identified an apoptotic program in gastrulation stage Xenopus embryos (Anderson, J.A., Lewellyn, A.L., Maller, J.L., 1997. Mol. Biol. Cell 8, 1195-1206; Stack, J.H., Newport, J.W., 1997. Development 124, 3185-3195). Here, we characterize in detail this maternal cell death program, which is set up at fertil...

journal_title：Mechanisms of development

authors： Hensey C,Gautier J

更新日期：1997-12-01 00:00:00

abstract：:The process of gastrulation is a pivotal step in the formation of the vertebrate body plan. The primary function of gastrulation is the correct placement of precursor tissues for subsequent morphogenesis. There is now mounting evidence that the body plan is established through inductive interactions between germ layer...

journal_title：Mechanisms of development

authors： Tam PP,Behringer RR

更新日期：1997-11-01 00:00:00

abstract：:During heart development, cells of the endocardial cushions undergo an epithelial-mesenchymal transformation and migrate into the surrounding extracellular matrix. This event is required for the normal formation of valves and chamber septation. Coincident with this phenotypic change is the expression of the serine pro...

journal_title：Mechanisms of development

authors： Majka SM,McGuire PG

更新日期：1997-11-01 00:00:00

abstract：:There has been rapid progress recently in the identification of signalling pathways regulating tooth development. It has become apparent that signalling networks involved in Drosophila development and development of mammalian organs such as the limb are also used in tooth development. Teeth are epithelial appendages f...

journal_title：Mechanisms of development

authors： Thesleff I,Sharpe P

更新日期：1997-10-01 00:00:00

abstract：:The recessive mutant mouse jumonji (jmj), obtained by a gene trap strategy, shows neural tube defects in approximately half of homozygotes with a Balb/cA and 129/Ola mixed background. Here, we show that no neural tube defects are observed with a Balb/cA background. We also found hypoplasia of the liver, thymus and spl...

journal_title：Mechanisms of development

authors： Motoyama J,Kitajima K,Kojima M,Kondo S,Takeuchi T

更新日期：1997-08-01 00:00:00

abstract：:We have generated a null allele of the mouse Msx1 homeobox gene by insertion of an nlacZ reporter gene into its homeobox. The sensitivity of beta-galactosidase detection permitted us to reveal novel aspects of Msx1 gene expression in heterozygous embryos, in particular in ectoderm and mesoderm during gastrulation, and...

journal_title：Mechanisms of development

authors： Houzelstein D,Cohen A,Buckingham ME,Robert B

更新日期：1997-07-01 00:00:00

abstract：:In the developing eye of Drosophila the protein kinase D-Raf controls the specification of the R7 photoreceptor cells. We show that overexpression of wild-type D-Raf inhibits the formation of R7 cells in a dose-dependent manner. Conversely, overexpression of mutant D-Raf proteins in which the conserved S388 is replace...

journal_title：Mechanisms of development

authors： Rommel C,Radziwill G,Moelling K,Hafen E

更新日期：1997-06-01 00:00:00

abstract：:Retinoic acid plays important roles in development, growth and differentiation by regulating the expression of target genes. A new retinoic acid-inducible gene, Stra6, has been identified in P19 embryonal carcinoma cells using a subtractive hybridization cDNA cloning technique. Stra6 codes for a very hydrophobic membr...

journal_title：Mechanisms of development

authors： Bouillet P,Sapin V,Chazaud C,Messaddeq N,Décimo D,Dollé P,Chambon P

更新日期：1997-05-01 00:00:00

abstract：:We describe the structural organization of a gene, termed XFDL 141/156, that is transiently activated during early Xenopus development. XFDL 141/156 is first transcribed at the midblastula transition (MBT) and during early gastrulation events. A roughly 200 nucleotide fragment immediately 5' to the transcription start...

journal_title：Mechanisms of development

authors： Bellefroid E,Bourguignon C,Bouwmeester T,Rausch O,Blumberg B,Pieler T

更新日期：1997-04-01 00:00:00

abstract：:The effects of signal perturbation on expression domains of molecular markers for the mesoderm and ectoderm have been analysed across the dorso-ventral axis in zebrafish embryos. Injection of RNA encoding bone morphogenetic protein-4 (BMP-4) ventralised the embryo, expanding the intermediate mesoderm and non-neural ec...

journal_title：Mechanisms of development

authors： Neave B,Holder N,Patient R

更新日期：1997-03-01 00:00:00